IndexMethods and MaterialsResultsDiscussionConclusionThe growing number of individuals seeking a wolfdog crossbreed as a pet has led to multiple research studies on wolfdog behavior. Wolves have a natural need to avoid human contact, in contrast, dogs crave attention. Mixing these two organisms has been found to create unpredictable behavioral responses among offspring and can pose a threat to livestock. However, the combination of wolf and dog genes in nature is not well studied, and the amount of livestock losses due to dog-wolf crossbreeding can only be predicted. Here I evaluate differences in offspring behavior in a single wolfdog litter, the amount of livestock losses in the United States, and dispersal patterns of wolfdog crossbreeds in Europe and Asia. Evidence of wolves and dogs interbreeding in the wild has been found across Europe and Asia, raising concern due to the unpredictable behavior observed between siblings in Mission: Wolf. According to government findings, the extent of livestock losses in the United States due to canines provides further evidence of concern. The combination of all these factors confirms a potential risk for cattle. The apparent variation in the behavior of individual wolfdogs and their ability to travel large distances can lead to the loss of livestock. Say no to plagiarism. Get a tailor-made essay on "Why Violent Video Games Shouldn't Be Banned"? Get an Original Essay Wolfdogs are desired by many people who are looking for a pet that possesses the personality of a domestic dog but the appearance of a wolf. Over 250,000 wolfdogs are born in the United States each year (White, 2001). Unfortunately, 80% of these “pets” will likely be killed before their third birthday (White, 2001). This is because the mixture of wolf and dog genes results in unpredictable behavioral patterns. The increased desire to have a wolfdog as a pet has opened the door to multiple genetic studies on how the mixture of genes influences behaviors. Although it has been found that interbreeding the two different animals creates a random distribution of behaviors in the offspring, the effects this may have on wolf-dog interactions in the wild are not well known. The research presented focuses on the effects of wolfdog genes on behavior, their occurrence in the wild, and livestock loss in the United States to canids. Loss of livestock due to predation from any source raises concern from conservationists, livestock owners, ranchers and the general public. When a pet is killed by a wolf, the media almost always draws attention to the matter. While truly wild wolves tend to stay away from livestock due to livestock's association with humans, dogs, on the other hand, are not as afraid of attacking livestock (Mech and Boitani, 2010). Crossing wolf and dog DNA can potentially create a wolf that isn't afraid to attack even livestock. This will cause truly wild wolves to develop a bad reputation. My goal is to show whether mixing wolf and dog genetic material creates unstable behavioral patterns that could in turn increase the amount of livestock losses due to wolves in the United States. Methods and Materials The study area of ​​this project, focusing primarily on the behavior of the North American Timber Wolf, is drawn from multiple studies conducted in the United States with an additional study of genetic material sampled from Eurasian wolves. In the complex we observe a species of wolf, Canis lupus,and the domestic dog, Canis lupus domesticus. Therefore, location differences do not affect the genetic composition of the two subjects. Further research on location-based behavioral differences is considered for this study, but is not well known. To understand the behavior of wolf-dog crosses, behavioral data was collected firsthand from Mission: Wolf, a wolf sanctuary located in the humid mountains of Colorado. The behavior of nine different wolfdogs was observed. Each wolfdog has its own distinct personality. The focus was on the personalities of the sanctuary's pit bull wolves: Buku, Oreo, Batman and Asia. All four were raised in the same environment and are a cross between a female gray wolf ("Valley Spirit") and an American pit bull, eliminating potential breed-related factors by allowing a direct comparison of the individuals' behaviors. However, a small sample size introduces sample bias, and future studies should include a larger sample population. For evidence to support my hypothesis, I will use a report from the National Agricultural Statistics Service (NASS). Based on the information contained in the report it is possible to calculate the total loss of livestock due to carnivore predation. This publication reports the deaths of 3.8 million cattle, or 4% of all livestock in the United States. Additionally, research on the abundance of dog genes in wild wolf populations of Europe and Asia is presented. LAMP, Admixture and Structure analyses, by author Małgorzata Pilot and colleagues, as well as the analysis of 61,000 single nucleotide polymorphisms (SNPs) in canid genomes are compared with results from dogs to determine the amount of dog-wolf admixture and the total amount of wolves versus wolfdogs in wild populations (Pilot et al., 2018). Results The behavior of pit bull wolves at Mission: Wolf varied between individuals. The Buku and Oreo puppies showed no aversion to human presence, a behavior characteristic of domestic dogs; However, as maturity increased, more typical wolf behaviors became evident, manifested in barking in fear in the presence of humans. The other individuals showed no revulsion towards humans, with Asia and Valley Spirit actively demanding further attention. This demonstrates case-by-case variation in the personality and behavior of wolfdog individuals and suggests that this may also be the case for wild wolfdogs. According to data collected by the National Agricultural Statistics Service (NASS) in 2011. As can be seen in Figure 1, carnivore-related deaths represented 0.2% of livestock losses, corresponding to approximately 216,000 of the 94 million head of livestock. livestock living in the United States. Of the 216,000 cattle killed, 3.7% (8,100) were victims of wolves, while 9.9% (21,800) were killed by domestic dogs. Therefore, dogs kill about three times more livestock than wolves. The introduction of dog alleles into wolf populations, whether intentionally or through natural crossbreeding in the wild, leads to unpredictable behavior that could result in even greater livestock losses. (National Agricultural Statistics Service, 2011) Figure 2 shows the geographic distribution of wolves and wolf-dog crossbreeds sampled in Europe and Asia by Małgorzata Pilot and colleagues. The black stars represent samples containing wolfdog mixes, with red dots showing pure wolf sampling and the pink shaded area showing the overall possible wolf range. The LAMP software in combination with mixture and structure analyzes has.