IndexIntroductionHypothesisMethodConclusionIntroductionBacteria are everywhere around us despite the fact that we are unaware of their existence. It's well known that bacteria exist, but it's easy for people to ignore this fact since we can't actually see the growth buildup in the surrounding areas. The colonies of bacteria that spread vary depending on the location and often tend to be more numerous in larger traffic areas. In this experiment the growth and removal of bacteria will be studied. Say no to plagiarism. Get a tailor-made essay on "Why Violent Video Games Shouldn't Be Banned"? Get an Original EssayThese high-traffic areas are often used almost tirelessly throughout the day, as multiple people often use certain areas of the home during their day routine. Kitchens and bathrooms tend to be the areas in our homes where we watch out for bacteria buildup, due to the various foods and transmission that occurs in these places. This begs the question: How can people ensure they rid themselves of bacterial growth? According to an article published in 2018 by author Erika Rawes, kitchens and bathrooms tend to have more bacterial growth than most places in a home. Door knobs, kitchen and bathroom sinks and faucets, refrigerator doors, bathroom floors and latrines appear to have the most bacterial growth due to the nature of their use. Kim Carollo explains that homes with pets also increase the varieties of bacteria that grow inside their homes. Carollo also emphasizes the importance of using appropriate cleaning products because some bacteria can make people in the house sick, or you can find overwhelming odors in different areas due to bacterial clusters. (Carollo, Kim. “Dirty Dogs: Homes with Pooches Loaded with Bacteria.”) Hypothesis The bathroom floor next to the toilet was thought to be prone to being an area where bacteria could accumulate. Some cleaning products may be more effective at removing bacteria; However, with so many cleaning products available on the market, it's difficult to know which will be most effective. Three chemicals were chosen for this experiment: Lysol disinfectant wipes with hydrogen peroxide, Scrubbing Bubbles bathroom cleaner, and isopropyl alcohol. Lysol wipes with hydrogen peroxide are believed to be the most effective chemical for removing bacteria around the toilet. I believe that the hydrogen peroxide agent will increase the likelihood of decreasing bacteria, as it is often used to kill bacteria by annihilating cell walls. The Lysol product also claims to remove 99.9% of bacteria. Of the three chemicals, I believe that isopropyl alcohol will remove the least amount of bacteria, as it is generally not used exclusively as a cleaning product and does not contain chemicals that kill the most bacteria. It is mainly used to kill surface bacteria for first aid purposes, but it evaporates quickly and will not have the same result as Lysol or Scrubbing Bubbles. Method The independent variable in this experiment is the active ingredients found in: Lysol Wipes (hydrogen peroxide) Bathroom Cleaner Scrubbing Bubbles (alkyl dimethyl benzyl ammonium chloride) and 70% isopropyl alcohol compound. These variables were used to define which detergent would be the most effective in eradicating the bacteria. Three isolated spots were selected on the floor and around the toilet with surfaces that appeared equally dirty. To ensure that each position was equal to obtainaccurate results, each area was swabbed, diluted with distilled water, and placed in three separate aerobic culture plates that were labeled prior to swabbing. Next, a sterile loop was used to streak the three agar plates before incubation. The cultures were subsequently placed in an incubator for 48 hours and observed during this period. The dependent variable was determined using the chemicals mentioned above, namely: disinfectant wipes, bubble scrubbing and isopropyl alcohol, to clean the three positions chosen and previously swabbed. Control area 1 was cleaned with Scrubbing Bubbles bathroom cleaner. The area was sprayed with Scrubbing Bubbles and scrubbed using a clean rag for exactly 60 seconds. A swab of the freshly cleaned area was taken and placed in the aerobic agar plate. This method was repeated for LysolWipes and isopropyl alcohol and no dilution was performed for any of these samples. Three new aerobic agar swabs and plates were used for this phase of the experiment. Each agar plate was labeled accordingly and then streaked using a sterile loop before being placed in an incubator for 48 hours. Growth of the dependent variable was monitored during the 48-hour time frame. This will subsequently be measured using colony forming units to determine the difference in bacterial growth before and after cleaning. Colony forming units are a method used to measure different types of cells, in this case bacterial cells, that originated from one type of bacteria. It is used to measure cellular bacterial infestation in agar plates. To obtain an accurate count of bacteria growing on the agar plate samples before and after cleaning, CFU was used and was calculated by counting the bacterial colonies on each plate, multiplying them by the sample dilution factor, and then multiplying it by the plating dilution factor. For control samples, the sample dilution factor is 100 and the plating dilution factor is 1. If bacterial growth covers all or most of the agar plate, it is counted as >1,000 colonies, which was the case of control. plate no. 1. If bacterial growth covers at least ¾ of the agar plate, then 1,000 colonies are counted, this was the case for control plates 2 and 3. Since only a few bacteria grew after cleaning the tested areas, these were counted individually . A bacterial colony can be identified in groups and often resemble small circles. Each circle is counted separately and visually, to obtain the number. The same CFU calculation was used to count the growth of bacteria in the agar plates comprising the samples from the cleaned areas. The confounding external variable was the possibility that different types of bacteria were growing in the three separate locations. This was organized by ensuring that all areas had the same types of bacteria. After the cultures remained in the incubator for 48 hours, they were removed to see if they were growing the same types of bacteria. To do this, colonies from each plate were smeared onto three separate slides, then allowed to air dry briefly before being placed on a hot plate for a few seconds and then peeled off. Subsequently, each slide was observed under a microscope and it was discovered that they all contained the same type of bacteria. The only difference between the three slides was the amount of bacteria. The amount of bacteria on each slide is determined visually under the microscope by counting each.
tags