The effect of substrate concentration on the activity of the enzyme catalaseA Level Biology ProjectObjectivesThis is an experiment to examine how the concentration of substrate hydrogen peroxide affects on the rate of reaction of the enzyme catalase.IntroductionThis is a genuine A-level school project and as such is intended for educational or research purposes only. Excerpts from this project should not be included in any project submitted for evaluation. This could lead to disqualification from all subjects you are taking. You have been warned. If you want more help doing your biology practice, take a look at "Advanced Level Practical Work for Biology" by Sally Morgan. If you want more detailed biological information, I recommend the book "Advanced Biology" by M. Kent. Objectives Basic information Apparatus Method Prediction Results Interpretation Limitations Anomalies Extension work Basic information Enzymes such as catalase are protein molecules found in living cells. They are used to accelerate specific reactions in cells. They are all very specific as each enzyme only performs one particular reaction. Catalase is an enzyme found in foods such as potatoes and liver. It is used to remove hydrogen peroxide from cells. Hydrogen peroxide is the poisonous byproduct of metabolism. Catalase accelerates the decomposition of hydrogen peroxide into water and oxygen, as shown in the equations below. Formula: Catalase Hydrogen Peroxide -----------------------> Water + Oxygen Catalase 2H2O2- ---->2H2O+O2It can accelerate decomposition of hydrogen peroxide because...... middle of paper......ental error involving one of the factors mentioned above. Extension work This experiment could be improved in several ways. It may be repeated several times to help eliminate any anomalies. A better overall result would be obtained by repeating the experiment several times because any errors in one experiment would have to be compensated for by the other experiments. Using higher concentrations of hydrogen peroxide would have produced a better graph and I would have liked to use higher concentrations above 20% to extend the graph to achieve the highest possible reaction rate. The problem of the delay between pouring the hydrogen peroxide, plugging the tube, and starting the stopwatch could have been limited by having another person start the stopwatch when the hydrogen peroxide was poured into the tube. Other biology projects