Advantages of materials Natural silk, collagen, gelatin, fibrinogen, hyaluronic acid, alginate • Biodegradable • Easily available • Bioactive, interacts with cells Synthetic PEG, PGA, PMMA, PLGA • Facilitates restoration of the structure of damaged tissues • Inert • Long lasting of preservation• Easily adaptable to the desired porosity and degradation time• Predictable and reproducible mechanical and physical propertiesTable 1. Natural and synthetic materials (Anandika Dhaliwal,2013)Application on 3D model contributing to oral cancerThree-dimensional (3D) in Cell culture in in vitro is the best system to reflect or mimic the behavior of tumor cells in vivo and their progression in the tumor microenvironment (Gurski et al., 2010). As before, cancer research depends on 2D model and small animal test model to study tumor angiogenesis, invasion, and metastasis (Godugu et al., 2013). However, the animal model is not ideal for long-term, large-scale research, although it responds with a more accurate tumor environment. Furthermore, 3D cell culture is advantageous over 2D cell culture system since the 2D model cannot fully study the cell-cell interaction during tumor progression as the 3D model does. Removal of tiny pieces of animal or human cancer tissue or obtained from the cell line is required in the 3D model where the tissue cell will interact with the organized matrix of the 3D model and express the similar cell behavior (Godugu et al., 2013). The 3D model also requires precise considerations on cell density, composition of the culture surface, culture medium used, supplements such as growth factor, pH, serum and oxygen where all of these can influence proliferation, differentiation, migration and apoptosis mobile phone. In addition to commercial availability... half the paper... technology nowadays improves the results of cancer research. The scientist sought to find an alternative way to understand the biology of cancer and then use it to help test the efficacy and molecular mechanisms of new and existing drugs to treat cancer patients. Integrated two-dimensional (2D) and three-dimensional (3D) systems have significantly increased cancer detection for both screening services. So 3D fulfills Cancer's desires whereby they have the ability to mimic Cancer's nature. It is useful for us to understand more about cells and the cell-matrix interaction that occurs during tumor growth and invasion. It can also reduce the rate of false positive results, as seen with cancer screening. Improving the effectiveness of the system already applied to prevent localized and widespread cancer.